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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
27/11/2008 |
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Data da última atualização: |
02/08/2019 |
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Autoria: |
MORAIS-LINO, L. S.; SEREJO, J. A. dos S.; SILVA, S. de O. e; SANTANA, J. R. F. de; KOBAYASHI, A. K. |
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Afiliação: |
Lucymeire Souza Morais-Lino, Universidade Estadual de Feira de Santana; JANAY ALMEIDA DOS SANTOS SEREJO, CNPMF; Sebastião de Oliveira e Silva; José Raniere Ferreira de Santana, Universidade Estadual de Feira de Santana; ADILSON KENJI KOBAYASHI, CPAMN. |
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Título: |
Cell suspension culture and plant regeneration of a Brazilian plantain, cultivar Terra. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 10, p. 1325-1330, out. 2008. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Cultura de células em suspensão e regeneração de plantas de bananeira cultivar Terra. |
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Conteúdo: |
The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male flowers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in the MS medium, with 11.4 µM indolacetic acid and 2.2 µM 6-benzylaminopurine. Regenerated plants showed a normal development, and no visible somaclonal variation was observed in vitro. It is possible to regenerate plants from cell suspensions of plantain banana cultivar Terra using MS medium supplemented with 11.4 µM of IAA and 2.2 µM of BAP. |
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Palavras-Chave: |
embriogênese somática; propagation. |
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Thesagro: |
Meio de Cultura; Micropropagação. |
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Thesaurus Nal: |
culture media; Musa; somatic embryogenesis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE-2009-09/44973/1/43n10a10.pdf
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Marc: |
LEADER 01874naa a2200265 a 4500
001 1122219
005 2019-08-02
008 2008 bl uuuu u00u1 u #d
100 1 $aMORAIS-LINO, L. S.
245 $aCell suspension culture and plant regeneration of a Brazilian plantain, cultivar Terra.
260 $c2008
500 $aTítulo em português: Cultura de células em suspensão e regeneração de plantas de bananeira cultivar Terra.
520 $aThe objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male flowers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in the MS medium, with 11.4 µM indolacetic acid and 2.2 µM 6-benzylaminopurine. Regenerated plants showed a normal development, and no visible somaclonal variation was observed in vitro. It is possible to regenerate plants from cell suspensions of plantain banana cultivar Terra using MS medium supplemented with 11.4 µM of IAA and 2.2 µM of BAP.
650 $aculture media
650 $aMusa
650 $asomatic embryogenesis
650 $aMeio de Cultura
650 $aMicropropagação
653 $aembriogênese somática
653 $apropagation
700 1 $aSEREJO, J. A. dos S.
700 1 $aSILVA, S. de O. e
700 1 $aSANTANA, J. R. F. de
700 1 $aKOBAYASHI, A. K.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 43, n. 10, p. 1325-1330, out. 2008.
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Embrapa Unidades Centrais (AI-SEDE) |
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